File:Late cytokinesis of Arabidopsis primary root meristem cell (two phragmoplasts and nuclei).tif
Late_cytokinesis_of_Arabidopsis_primary_root_meristem_cell_(two_phragmoplasts_and_nuclei).tif (512 × 512 pixels, file size: 411 KB, MIME type: image/tiff)
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Summary[edit]
DescriptionLate cytokinesis of Arabidopsis primary root meristem cell (two phragmoplasts and nuclei).tif |
English: Сonfocal image of mitotic microtubules (phragmoplasts; green signal from anti-tubulin antibody conjugated with Alexa Fluor 488) and nuclei (blue signal from DNA stained with DAPI) in root meristem cells 4-day old Arabidopsis thaliana (Col-0) visualized by whole-mount tubulin immunolabeling (Komis and Šamaj, 2014). Image was documented with a Zeiss 710 confocal laser scanning microsope (CLSM) platform (Carl Zeiss, Germany) at the Centre of the Region Haná for Biotechnological and Agricultural Research, (Olomouc, Czech Republic) by the research fellow of the "Institute of Food Biotechnology and Genomics of National Academy of Sciences of Ukraine" (Kyiv, Ukraine). Laser line used for GFP exitation – Ar 488 nm, for DAPI – 405 nm. The sum image represents maximum intensity projection of Z-stacks series reconstructed in Zeiss Zen 2012 software. Scale bar: 5 um. |
Date | |
Source | Own work |
Author | Yuliya Krasylenko |
Конфокальна мікрофотографія мітотичних мікротрубочок (фрагмопласти; зелений сигнал від анти-тубуліну, кон’югованого з барвником Alexa Fluor 488) та ядра (ДНК; блакитний сигнал від DAPI) у клітинах меристеми головних коренів чотириденних проростків Arabidopsis thaliana (Col-0), візуалізованих за допомогою техніки whole-mount tubulin immunolabeling (Komis and Šamaj, 2014). Зображення отримано за допомогою лазерного скануючого конфокального мікроскопа Zeiss 710 (Carl Zeiss, Germany) у Центрі біотехнологічних та сільськогосподарських досліджень регіону Хана (м. Оломоуц, Чеська Республіка) науковим співробітником ”Інститут харчової біотехнології та геноміки НАН України” (м. Київ, Україна). Для збудження флюорофору GFP використано аргоновий лазер з довжиною хвилі 488 нм. Сумарне зображення являє собою накладання серії оптичних зрізів (так званих Z-стеків). Масштаб мікрофотографії: 5 мкм.
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current | 23:24, 28 October 2016 | 512 × 512 (411 KB) | Yuliya Krasylenko (talk | contribs) | User created page with UploadWizard |
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Width | 512 px |
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Height | 512 px |
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Compression scheme | LZW |
Pixel composition | RGB |
Number of components | 3 |
Number of rows per strip | 5 |
Horizontal resolution | 72 dpi |
Vertical resolution | 72 dpi |
Data arrangement | chunky format |