File:The-Human-Polyoma-JC-Virus-Agnoprotein-Acts-as-a-Viroporin-ppat.1000801.s008.ogv

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The-Human-Polyoma-JC-Virus-Agnoprotein-Acts-as-a-Viroporin-ppat.1000801.s008.ogv ‎(Ogg Theora video file, length 9.1 s, 180 × 110 pixels, 89 kbps, file size: 99 KB)

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English: HeLa cells transfected with pERedNLS-Agno and FRET probe (YC3.60). Cells coexpressing pERedNLS-Agno (Video S1) or pERedNLS (Video S2) and YC3.60 were incubated for 72 h. Permeability to Ca2+ in agnoprotein-expressing (Video S1) or mock-transfected (Video S2) cells was evaluated by the change in FRET ratio induced by the addition of 5 mM CaCl2 to the extracellular medium 100 sec after the start of recording. Fluorescent images of CFP and FRET were recorded every 10 sec for 15 min. Ratio images of FRET/CFP were created to represent FRET efficiency. In the IMD mode shown here, eight colors from red to blue are used to represent the FRET/CFP ratio. The upper (red) and lower (blue) limits of the ratio range are 4.5 and 2.6, respectively.
Date
Source Video S1 from Suzuki T, Orba Y, Okada Y, Sunden Y, Kimura T, Tanaka S, Nagashima K, Hall W, Sawa H (2010). "The Human Polyoma JC Virus Agnoprotein Acts as a Viroporin". PLOS Pathogens. DOI:10.1371/journal.ppat.1000801. PMID 20300659. PMC: 2837404.
Author Suzuki T, Orba Y, Okada Y, Sunden Y, Kimura T, Tanaka S, Nagashima K, Hall W, Sawa H
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Date/TimeThumbnailDimensionsUserComment
current13:31, 17 November 20129.1 s, 180 × 110 (99 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 31 kbps Completed 17:02, 17 December 2023 1.0 s
WebM 360P 41 kbps Completed 15:37, 2 December 2023 1.0 s
Streaming 144p (MJPEG) 270 kbps Completed 11:42, 9 November 2023 1.0 s

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