File:Redistribution-of-Actin-during-Assembly-and-Reassembly-of-the-Contractile-Ring-in-Grasshopper-pone.0004892.s005.ogv

English: Redistribution of actin filaments labeled by Qdot 655-Phalloidin. Video S5 corresponds to Figure 4. Actin filaments were labeled by microinjection of phalloidin-conjugated Qdot 655 into a spermatocyte. At the onset of cytokinesis, the Qdot-decorated actin filaments formed a barely distinct pattern, vaguely reminiscent of the distribution of central spindle microtubules. (Note that the spindle-like pattern was unavoidably superimposed on a cortical flow-derived pattern, as the Qdots can label cortical actin filaments as well as cytoplasmic filaments.) Qdots gradually accumulated at the cleavage furrow as cytokinesis proceeded. They were largely excluded from the region of the segregated chromosomes (dark spot in the center of each nascent daughter cell).