File:MiR-34a-Targeting-of-Notch-Ligand-Delta-Like-1-Impairs-CD15+CD133+-Tumor-Propagating-Cells-and-pone.0024584.s010.ogv

English: Tumor spheres isolated from Patch1+/- P53-/- mice infected with AdV-miR-34a show sign of induction of differentiation only when they are not dissociated before the infection. At this time, the infected cells remain in contact with each other and are subjected to Notch signaling via the cell non-autonomous pathway. A time-series of phase-contrast brightfield images of the medullpspheres were acquired with an A-Plan Ph1 10× objective for a total time of 72 h, using a Zeiss Axiovert 200M microscope equipped with an Okolab WJ CO2 Microscope Stage Incubator, for controlling the temperature and the %CO2. The time delay between individual images used here was 15 min.