File:Membrane-Bound-IL-21-Promotes-Sustained-Ex-Vivo-Proliferation-of-Human-Natural-Killer-Cells-pone.0030264.s002.ogv

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Membrane-Bound-IL-21-Promotes-Sustained-Ex-Vivo-Proliferation-of-Human-Natural-Killer-Cells-pone.0030264.s002.ogv ‎(Ogg Theora video file, length 10 s, 700 × 494 pixels, 628 kbps, file size: 767 KB)

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English: Video fluorescent microscopy of NK cell lysis of neuroblastoma targets. CHP134 neuroblastoma targets were loaded with calcein dye as described. NK cells were added to target cells at a 5∶1 E∶T ratio, and fluorescent and brightfield images were acquired at 4-minute intervals using the Nikon BioStation. Sudden loss of fluorescence indicates a loss of cell membrane integrity associated with perforin release by NK cells. Total duration of the movie file spans 2 hours 40 minutes.
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Source Movie S1 from Denman C, Senyukov V, Somanchi S, Phatarpekar P, Kopp L, Johnson J, Singh H, Hurton L, Maiti S, Huls M, Champlin R, Cooper L, Lee D. "Membrane-Bound IL-21 Promotes Sustained Ex Vivo Proliferation of Human Natural Killer Cells". PLOS ONE. DOI:10.1371/journal.pone.0030264. PMID 22279576. PMC: 3261192.
Author Denman C, Senyukov V, Somanchi S, Phatarpekar P, Kopp L, Johnson J, Singh H, Hurton L, Maiti S, Huls M, Champlin R, Cooper L, Lee D
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This file is licensed under the Creative Commons Attribution 2.5 Generic license.
You are free:
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Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
Copyright owner: Denman et al.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current03:14, 18 October 201210 s, 700 × 494 (767 KB)Open Access Media Importer Bot (talk | contribs)Uploaded with the Open Access Media Importer. (test edit) botrequest

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Format Bitrate Download Status Encode time
VP9 480P 226 kbps Completed 06:08, 3 September 2018 4.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 149 kbps Completed 06:08, 3 September 2018 3.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 92 kbps Completed 06:08, 3 September 2018 3.0 s
Streaming 240p (VP9) 92 kbps Completed 16:56, 2 February 2024 2.0 s
WebM 360P 488 kbps Completed 05:27, 20 November 2012 5.0 s
Streaming 144p (MJPEG) 157 kbps Completed 01:51, 12 November 2023 1.0 s

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