File:Investigating-the-Effect-of-Emetic-Compounds-on-Chemotaxis-in-Dictyostelium-Identifies-a-Non-pone.0024439.s002.ogv

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Investigating-the-Effect-of-Emetic-Compounds-on-Chemotaxis-in-Dictyostelium-Identifies-a-Non-pone.0024439.s002.ogv ‎(Ogg Theora video file, length 15 s, 278 × 208 pixels, 642 kbps, file size: 1.15 MB)

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English: Dictyostelium chemotaxis was monitored by time-lapse photography to record cells moving within a Dunn Chamber towards the chemo-attractant, cAMP, across the screen from bottom right to top left. Images were taken every 6 seconds over a 15 minute period. Computer generated cell outlines enables average cell velocity, shape and direction of movement to be quantified. Cell movement was recorded over a 5 minute period, prior to the addition a range of emetic or aversive compounds (indicated by a black screen flash), and cell behaviour was recorded for a further 10 minutes. In the movie shown here, the stomach irritant copper sulphate (5 mM ) was added, causing Dictyostelium cells to slowly stop moving and lose shape over the ten min period tested.
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Source Movie S2 from Robery S, Mukanowa J, Percie du Sert N, Andrews P, Williams R (2011). "Investigating the Effect of Emetic Compounds on Chemotaxis in Dictyostelium Identifies a Non-Sentient Model for Bitter and Hot Tastant Research". PLOS ONE. DOI:10.1371/journal.pone.0024439. PMID 21931717. PMC: 3169598.
Author Robery S, Mukanowa J, Percie du Sert N, Andrews P, Williams R
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Date/TimeThumbnailDimensionsUserComment
current11:45, 18 November 201215 s, 278 × 208 (1.15 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 222 kbps Completed 06:20, 6 December 2023 5.0 s
WebM 360P 262 kbps Completed 15:57, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 627 kbps Completed 21:42, 15 November 2023 1.0 s

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