File:Escaping-Underground-Nets-Extracellular-DNases-Degrade-Plant-Extracellular-Traps-and-Contribute-to-ppat.1005686.s006.ogv

English: R . solanacearum Δ nucA/B double nuclease mutant cells were released from trapping by pea root border cell NETs after purified NucA was added. 107 mutant bacterial cells were incubated with approximately 10,000 border cells from 2-day old pea seedlings at room temperature for 24h. Ten μg/ml of purified NucA was added and the cells were incubated at room temperature for an additional hour. The release of trapped bacteria was monitored at 400X magnification, using a Leica DM LB light microscope equipped with a Dino AM-4023XC camera.