File:Dual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv004.ogv
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DescriptionDual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv004.ogv |
English: Time-Lapse Microscopy of S2 cells Stably Expressing CID-GFP and RFP-H2B and Depleted for TOPO II, 96 h after the Addition of the dsRNA Z-stacks are composed of ten optical sections covering 10 μm and were acquired every 15 s. Anaphase onset corresponds to time 0 s. Merge color images for H2BRFP (red) and CID-GFP (green) is shown on the left. The separate channel for CID-GFP (white) can be seen on the right. Pairs of centromeres were identified and labeled in each optical section of the Z-stack as described above. Pairs of the centromere marker CID-GFP are labeled with the same color. |
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Source | Video S4 from Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C (2008). "Dual Role of Topoisomerase II in Centromere Resolution and Aurora B Activity". PLOS Biology. DOI:10.1371/journal.pbio.0060207. PMID 18752348. PMC: 2525683. | ||
Author | Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C | ||
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current | 23:24, 30 October 2012 | 6.7 s, 1,056 × 312 (119 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Time-Lapse Microscopy of S2 cells Stably Expressing CID-GFP and RFP-H2B and Depleted for TOPO II, 96 h after the Addition of the dsRNA |
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Author | Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Z-stacks are composed of ten optical sections covering 10 ?m and were acquired every 15 s. Anaphase onset corresponds to time 0 s. Merge color images for H2BRFP (red) and CID-GFP (green) is shown on the left. The separate channel for CID-GFP (white) can be seen on the right. Pairs of centromeres were identified and labeled in each optical section of the Z-stack as described above. Pairs of the centromere marker CID-GFP are labeled with the same color. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-08 |
Categories:
- Videos of 2008
- Cell cycle proteins
- Videos of cultured cells of Drosophila melanogaster
- Centromere
- Non-histone chromosomal proteins
- Chromosome segregation
- Type II DNA topoisomerases
- Videos of Drosophila melanogaster proteins
- Enzyme activation
- Schneider 2 cells
- Kinetochores
- Videos of microtubules of the spindle apparatus
- Protein-serine-threonine kinases
- RNA interference
- Sister chromatid exchange
- Topoisomerase II inhibitors
- Aurora kinase B