File:Centrosome migration in a living Drosophila female interphase germline stem cell - pbio.1001357.s006.ogv

English: Centrosome migration in a living female interphase GSC. In this movie, one GSC centrosome is localized at the niche-GSC interface, while the other centrosome moves between 10° and 15° around the circumference of the GSC toward the posterior. Figure S2 contains representative frames from this movie. In this movie and in Movie S2, centrosomes are marked by a GFP-tagged pericentrosomal protein Centrosomin (GFP-Cnn, shown in green) under the control of germline-specific Nos-Gal4 driver. The nuclei of the somatic niche cells (and to a lesser extent, the undifferentiated anterior germline cells) are marked by a living-cell DNA dye (Draq5-Cy5, shown in red). The boundary of Cnn-GFP and bright Draq5 staining delineates the CpC-GSC interface. Each movie covers about 30 min of development, with one frame taken every minute. Each frame is a projection of a stack of eight 1 µm confocal slices. The images were taken using a 63× water lens of a DSU Olympus Spinning Disk Confocal Microscope (see more details in Text S1 ). (MOV)