File:Calcium-Dynamics-of-Cortical-Astrocytic-Networks-In-Vivo-pbio.0020096.sv005.ogv
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English: Double-Labeling of Fluo-4 AM-Loaded Astrocytes with S100B Antibody Acute slices (300 μm thickness) were prepared from the in vivo Fluo-4 AM-loaded somatosensory cortex. The slices were subsequently incubated in EDAC containing saline followed by formalin fixation. The loaded astrocytes were identified by examination at various depths and numbered (left). Next, the slices were processed for immunocytochemistry with astrocyte marker S100B. Depth scans (1 μm between the frames) were taken again to determine immunoreactivity of cells with S100B (right movie). An overlapping set of the cells was identified to be S100B-immunoreactive, indicating that nearly all Fluo-4 AM-loaded cells were astrocytes.
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| Source | Video S5 from Hirase H, Qian L, Barthó P, Buzsáki G (2004). "Calcium Dynamics of Cortical Astrocytic Networks In Vivo". PLOS Biology. DOI:10.1371/journal.pbio.0020096. PMID 15094801. PMC: 387267. | ||
| Author | Hirase H, Qian L, Barthó P, Buzsáki G | ||
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 01:12, 31 October 2012 | 14 s, 1,000 × 500 (2 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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