File:A-Volumetric-Method-for-Quantifying-Atherosclerosis-in-Mice-by-Using-MicroCT-Comparison-to-En-Face-pone.0018800.s007.ogv

English: 5-Panel Percent Occlusion Map Movie of Ldlr-1KO-AD aorta 4 (Table 1): This movie ties together the three-dimensional aortic arch with the segmentation and quantitative metrics. The movie is split into five panels; each panel is described below: 3D Rendering Frame (left): we display the 3D aortic arch, with a perpendicular slice. The arch is rendered as two surfaces: the vessel is shown in a transparent gray; the plaque is shown in dark yellow. At the bottom of this frame, we report the slice number and slice percent occlusion as the movie progresses (and indicated by the black ring), as well as total specimen measurements for the average occlusion, total plaque volume, and total attached surface area. Linear Distance Measurements (top-right): for each slice along the aorta, we graph the average aorta radius, maximum plaque thickness, and the average plaque thickness. As the animation proceeds, a black time-bar indicates the current slice position. Percent Measurements (middle-right): for each slice along the curved centerline, we graph the percent coverage (percent of the vessel wall that has plaque attached) and percent occlusion (percent of the vessel cross-section that is occluded with plaque). As the animation proceeds, a black time-bar indicates the current slice position. Planar Slice (bottom-middle): slice of microCT volume that cuts through and is centered on the aorta, and which is oriented perpendicular to the centerline. Planar Slice with Segmentation Overlay (bottom-right): planar slice (same as above) with plaque segmentation overlaid in red. Total Percent Occlusion: The total percent occlusion for each specimen is taken as the average of the percent occlusion values calculated at each centerline-reformatted slice. We note that this formulation is different than the value that results from the ratio of the total plaque volume to the total vessel volume (which we do not report).