File:Tyrosine-Binding-Protein-Sites-Regulate-the-Intracellular-Trafficking-and-Processing-of-Amyloid-pone.0161445.s002.ogv
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Tyrosine-Binding-Protein-Sites-Regulate-the-Intracellular-Trafficking-and-Processing-of-Amyloid-pone.0161445.s002.ogv (Ogg Theora video file, length 12 s, 482 × 463 pixels, 1.53 Mbps, file size: 2.17 MB)
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DescriptionTyrosine-Binding-Protein-Sites-Regulate-the-Intracellular-Trafficking-and-Processing-of-Amyloid-pone.0161445.s002.ogv |
English: APP is trafficked rapidly to lysosomes from the Golgi. SN56 cells were transiently transfected with βAPP-paGFP (green), LAMP1-mRFP (lysosome marker, red), and GalT-CFP (Golgi marker, blue). APP was photo-activated in the Golgi (blue) with 405nm light, alternating with imaging for 15 minutes (indicated by green word ‘Photo-activating’). The white circles appearing over the Golgi denote the initial ROIs for βAPP-paGFP photoactivation. These ROIs are carefully monitored and adjusted to remain on the Golgi during the photoactivation period. Cells were then chased imaging every 30 seconds for the indicated time. |
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Source | S1 Video from Tam J, Cobb M, Seah C, Pasternak S (2016). "Tyrosine Binding Protein Sites Regulate the Intracellular Trafficking and Processing of Amyloid Precursor Protein through a Novel Lysosome-Directed Pathway". PLOS ONE. DOI:10.1371/journal.pone.0161445. PMID 27776132. PMC: 5077117. | ||
Author | Tam J, Cobb M, Seah C, Pasternak S | ||
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 08:59, 31 January 2017 | 12 s, 482 × 463 (2.17 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | APP is trafficked rapidly to lysosomes from the Golgi. |
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Author | Tam J, Cobb M, Seah C, Pasternak S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | SN56 cells were transiently transfected with βAPP-paGFP (green), LAMP1-mRFP (lysosome marker, red), and GalT-CFP (Golgi marker, blue). APP was photo-activated in the Golgi (blue) with 405nm light, alternating with imaging for 15 minutes (indicated by green word ‘Photo-activating’). The white circles appearing over the Golgi denote the initial ROIs for βAPP-paGFP photoactivation. These ROIs are carefully monitored and adjusted to remain on the Golgi during the photoactivation period. Cells were then chased imaging every 30 seconds for the indicated time. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-10-24 |