File:Transcription-Factors-MYOCD-SRF-Mesp1-and-SMARCD3-Enhance-the-Cardio-Inducing-Effect-of-GATA4-TBX5-pone.0063577.s015.ogv
Transcription-Factors-MYOCD-SRF-Mesp1-and-SMARCD3-Enhance-the-Cardio-Inducing-Effect-of-GATA4-TBX5-pone.0063577.s015.ogv (Ogg Theora video file, length 30 s, 696 × 520 pixels, 485 kbps, file size: 1.72 MB)
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[edit]DescriptionTranscription-Factors-MYOCD-SRF-Mesp1-and-SMARCD3-Enhance-the-Cardio-Inducing-Effect-of-GATA4-TBX5-pone.0063577.s015.ogv |
English: Induced pluripotent stem cells derived from transgenic MEFs (Myh6.eGFP.Myh6.PAC) using a single inducible polycystronic lentiviral vector containing all four reprogramming factors (Oct4, Sox2, Klf4, Myc) were differentiated using the hanging droplet technique. Regions containing spontaneously contracting cardiomyocytes were readily detected as early as 7 days post initiation of differentiation. GFP expression was specific to the spontaneously contracting cells. |
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Source | Movie S1 from Christoforou N, Chellappan M, Adler A, Kirkton R, Wu T, Addis R, Bursac N, Leong K (2013). "Transcription Factors MYOCD, SRF, Mesp1 and SMARCD3 Enhance the Cardio-Inducing Effect of GATA4, TBX5, and MEF2C during Direct Cellular Reprogramming". PLOS ONE. DOI:10.1371/journal.pone.0063577. PMID 23704920. PMC: 3660533. | ||
Author | Christoforou N, Chellappan M, Adler A, Kirkton R, Wu T, Addis R, Bursac N, Leong K | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:36, 25 May 2013 | 30 s, 696 × 520 (1.72 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Christoforou N, Chellappan M, Adler A, Kirkton R, Wu T, Addis R, Bursac N, Leong K |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Induced pluripotent stem cells derived from transgenic MEFs (Myh6.eGFP.Myh6.PAC) using a single inducible polycystronic lentiviral vector containing all four reprogramming factors (Oct4, Sox2, Klf4, Myc) were differentiated using the hanging droplet technique. Regions containing spontaneously contracting cardiomyocytes were readily detected as early as 7 days post initiation of differentiation. GFP expression was specific to the spontaneously contracting cells. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |