File:The-immune-synapse-clears-and-excludes-molecules-above-a-size-threshold-ncomms6479-s2.ogv
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The-immune-synapse-clears-and-excludes-molecules-above-a-size-threshold-ncomms6479-s2.ogv (Ogg Theora video file, length 5.0 s, 219 × 219 pixels, 573 kbps, file size: 350 KB)
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DescriptionThe-immune-synapse-clears-and-excludes-molecules-above-a-size-threshold-ncomms6479-s2.ogv |
English: Supplementary Movie 1 Dextran sized 4 nm is not cleared from the synapse during assembly. Primary NK cells were plated with Texas Red-labelled 4 nm dextran and fluorescein-labelled 32 nm dextran. Synapse formation with the slide was imaged by time-lapse total internal reflection fluorescence microscopy and images were taken every 20 seconds. Video shows the 4 nm dextran at the cell-slide interface during synapse formation. Fluorescence intensity threshold shows greyscale images with pixel intensities below 32 AU (blue) and above 223 AU (red). Scale bar; 10 μm. The movie corresponds to Figure 4g (upper row). |
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Source | Video file from Cartwright A, Griggs J, Davis D (2014). "The immune synapse clears and excludes molecules above a size threshold". Nature Communications. DOI:10.1038/ncomms6479. PMID 25407222. PMC: 4366483. | ||
Author | Cartwright A, Griggs J, Davis D | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 05:48, 2 November 2016 | 5.0 s, 219 × 219 (350 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 1 |
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Author | Cartwright A, Griggs J, Davis D |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Dextran sized 4 nm is not cleared from the synapse during assembly. Primary NK cells were plated with Texas Red-labelled 4 nm dextran and fluorescein-labelled 32 nm dextran. Synapse formation with the slide was imaged by time-lapse total internal reflection fluorescence microscopy and images were taken every 20 seconds. Video shows the 4 nm dextran at the cell-slide interface during synapse formation. Fluorescence intensity threshold shows greyscale images with pixel intensities below 32 AU (blue) and above 223 AU (red). Scale bar; 10 μm. The movie corresponds to Figure 4g (upper row). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014-11-19 |