File:The-Diverse-and-Dynamic-Nature-of-Leishmania-Parasitophorous-Vacuoles-Studied-by-Multidimensional-pntd.0001518.s003.ogv

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The-Diverse-and-Dynamic-Nature-of-Leishmania-Parasitophorous-Vacuoles-Studied-by-Multidimensional-pntd.0001518.s003.ogv ‎(Ogg Theora video file, length 1 min 0 s, 364 × 320 pixels, 232 kbps, file size: 1.65 MB)

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English: Macrophage acidic vesicles decrease in number during L. amazonensis PV biogenesis and growth. (A–C) Separation of acidic vesicles from Leishmania PVs using the same fluorescence channel. (A) Multidimensional image of macrophages infected with L. amazonensis and loaded with Lysotracker. A window for software analysis was established to identify acidic vesicles exclusively in the selected macrophage. (B) Isospots 1 µm in diameter were attributed to Lysotracker channel voxels in multidimensional images. The software identified small vesicles surrounding L. amazonensis PVs but interpreted PVs as clusters of Lysotracker-positive vesicles. Each isospot has a statistic-based color corresponding to the mean Lysotracker RFI (colored bar). (C) By adjusting the thresholds of isospots detection based on Lysotracker RFI, the software can attribute isospots to the acidic vesicles (orange isospots in the second image, excluding the fluorescence signal of Leishmania PVs. Multidimensional acquisition started after 2 h of infection, and the time of acquisition is shown as hh:mm.
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Source Video S3 from Real F, Mortara R (2012). "The Diverse and Dynamic Nature of Leishmania Parasitophorous Vacuoles Studied by Multidimensional Imaging". PLOS Neglected Tropical Diseases. DOI:10.1371/journal.pntd.0001518. PMID 22348167. PMC: 3279510.
Author Real F, Mortara R
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current20:37, 18 November 20121 min 0 s, 364 × 320 (1.65 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 85 kbps Completed 18:18, 21 October 2018 17 s
Streaming 240p (VP9) 86 kbps Completed 16:20, 17 December 2023 1.0 s
WebM 360P 145 kbps Completed 15:26, 2 December 2023 4.0 s
Streaming 144p (MJPEG) 594 kbps Completed 10:31, 9 November 2023 1.0 s

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