File:Staphylococcal-Enterotoxin-A-Induces-Small-Clusters-of-HLA-DR1-on-B-Cells-pone.0006188.s004.ogv

English: Acquisition of Tilt series. A thick section of embedded and stained B cell (see Materials and Methods) was imaged at tilt angles ranging from −60° to +60° at 2° increments, cross-correlated and the individual images sequentially output as a movie. The gold fiducials appear as large dark dots, while the 5 nm gold markers which label the surface MHC class II appear as small dots on the cell membrane (the nucleus is on the bottom right). The latter are easier to visualize when the movie is allowed to play at normal speed, and appear most prominently at lower tilt angles, e.g. a group of gold beads near the centre of the image, to the top-left of the small spherical vesicle.