File:Premitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s007.ogv

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Original file ‎(Ogg Theora video file, length 22 s, 767 × 762 pixels, 766 kbps, file size: 1.99 MB)

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English: CENP-W knockdown induces profound mitotic delays and induces chromosome/spindle rolling in cells. HeLa-H2B-GFP cells were transfected with pooled siRNAs against CENP-W and imaged for 12 h beginning 48-h transfection at a rate of 20 frames/hour. Numerous cells are observed entering mitosis and spending extended time in a prometaphase-like state with uncongressed chromosomes. Many cells exhibit a pronounced “rolling” phenotype (see upper right quadrant) that can initiate very soon after the onset of mitosis. See Figure 2B for a quantitative interpretation of these data.
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Source Video S1 from Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K (2011). "Premitotic Assembly of Human CENPs -T and -W Switches Centromeric Chromatin to a Mitotic State". PLOS Biology. DOI:10.1371/journal.pbio.1001082. PMID 21695110. PMC: 3114758.
Author Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K
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Date/TimeThumbnailDimensionsUserComment
current22:25, 14 November 201222 s, 767 × 762 (1.99 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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