File:Plasticity-in-the-Macromolecular-Scale-Causal-Networks-of-Cell-Migration-pone.0090593.s007.ogv

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Original file ‎(Ogg Theora video file, length 8.2 s, 3,072 × 1,024 pixels, 3.98 Mbps, file size: 3.89 MB)

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English: Control cell migration. Control (DMSO-treated) H1299-P/L cells expressing EGFP-Paxillin (green, left panel) and RubyRed-LifeAct (red, left panel) were imaged at 5 min intervals for 8 h. EGFP-Paxillin is concentrated within punctate cell-matrix adhesion complexes (CMACs) while RubyRed-LifeAct associates with the F-Actin cytoskeleton. After raw confocal image acquisition (left panel), automated image analysis facilitated segmentation of individual cells based on RubyRed-LifeAct fluorescence (cell boundary indicated by dark blue outline, overlaying EGFP-Paxillin channel, center panel). Subsequently, CMACs within each cell were segmented (CMACs detected based on EGFP-Paxillin fluorescence, indicated by red outlines, center panel). Finally, the centroids of both cells and their constituent CMACs were tracked via nearest neighbor analysis to facilitate time-resolved analyses of dynamic migratory processes (right panel). CMAC trajectories are color-coded for time, ≤10 time points shown. Scale bars in 1st frame = 50 µm.
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Source Movie S1 from Lock J, Mamaghani M, Shafqat-Abbasi H, Gong X, Tyrcha J, Stromblad S (2014). "Plasticity in the Macromolecular-Scale Causal Networks of Cell Migration". PLOS ONE. DOI:10.1371/journal.pone.0090593. PMID 24587399. PMC: 3938764.
Author Lock J, Mamaghani M, Shafqat-Abbasi H, Gong X, Tyrcha J, Stromblad S
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current02:43, 7 March 20148.2 s, 3,072 × 1,024 (3.89 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 1440P 2.21 Mbps Completed 20:58, 13 October 2018 13 s
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