File:Phosphatidylinositol-(45)-Bisphosphate-Controls-T-Cell-Activation-by-Regulating-T-Cell-Rigidity-and-pone.0027227.s008.ogv

English: A representative interaction of sensor-transduced 5C.C7 T cell with a CH27 B cell lymphoma APC in the presence of 10 µM MCC agonist peptide is shown in this and all subsequent movies. Movies were acquired using time-lapse epifluorescence microscopy. Differential interference contrast (DIC) images are shown on top, with matching top-down, maximum projections of 3-dimensional sensor fluorescence data on the bottom. The sensor fluorescence intensity is displayed in a rainbow-like false-color scale (increasing from blue to red). 20 s intervals in movie acquisition are played back as 2 frames per second. The individual captions list for each movie the figure it refers to, the sensor and any non-fluorescent additional proteins expressed by the 5C.C7 T cell, the time of T cell/APC couple formation given both as the movie frame number and the displayed movie time (in parentheses), and noteworthy features of the movie. Movie S1 refers to Fig. S1C , the 5C.C7 T cells are transduced with PIP5K α-GFP, cell coupling occurs in frame 3 (1s), and prominent are a large lamellum upon cell coupling and transient interface accumulation of PIP5K α-GFP.