File:Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s010.ogv

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Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s010.ogv ‎(Ogg Theora video file, length 8.7 s, 222 × 240 pixels, 350 kbps, file size: 370 KB)

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English: Inhibition of PI3K activity blocked the PA-Rac1-induced formation of growth cone lamellipodia. Time-lapse images of a stage 2 mouse hippocampal neuron expressing both mCherry and PA-Rac1. mCherry fluorescence images were acquired every 10 s. The red circled area was continuously photoactivated (405-nm laser at 10% of maximal power, 0.1 Hz) from 0 min and LY294002 (100 µM) was applied at 10 min. LY294002 application blocked formation of growth cone lamellipodia induced by PA-Rac1 photoactivation and prevented growth cone motility. Selected images from this movie are shown in Fig. 7A.
Date
Source Movie S8 from Kakumoto T, Nakata T (2013). "Optogenetic Control of PIP3: PIP3 Is Sufficient to Induce the Actin-Based Active Part of Growth Cones and Is Regulated via Endocytosis". PLOS ONE. DOI:10.1371/journal.pone.0070861. PMC: 3737352.
Author Kakumoto T, Nakata T
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Date/TimeThumbnailDimensionsUserComment
current06:01, 18 August 20138.7 s, 222 × 240 (370 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 211 kbps Completed 02:59, 6 September 2018 5.0 s
Streaming 240p (VP9) 212 kbps Completed 21:43, 6 February 2024 1.0 s
WebM 360P 212 kbps Completed 03:51, 2 December 2023 1.0 s
Streaming 144p (MJPEG) 857 kbps Completed 13:20, 17 November 2023 1.0 s

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