File:Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s009.ogv

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Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s009.ogv ‎(Ogg Theora video file, length 8.0 s, 320 × 260 pixels, 496 kbps, file size: 484 KB)

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English: Local PIP3 production in the middle of the neurites caused “wave”. Time-lapse images of a stage 2 mouse hippocampal neuron expressing the PI3K photoswitch (plasmid #2). mCh-PHR-iSH images were acquired every 10 s. The red circled area was continuously photoactivated (405-nm laser at 10% of maximal power, 0.1 Hz) from 0 min. Photoactivation of the PI3K photoswitch induced accumulation of mCh-PHR-iSH. Then, lamellipodial protrusions formed at the photoactivation site and moved retrogradely. Selected images from this movie are shown in Fig. 6B.
Date
Source Movie S7 from Kakumoto T, Nakata T (2013). "Optogenetic Control of PIP3: PIP3 Is Sufficient to Induce the Actin-Based Active Part of Growth Cones and Is Regulated via Endocytosis". PLOS ONE. DOI:10.1371/journal.pone.0070861. PMC: 3737352.
Author Kakumoto T, Nakata T
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current06:01, 18 August 20138.0 s, 320 × 260 (484 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 211 kbps Completed 02:59, 6 September 2018 8.0 s
Streaming 240p (VP9) 212 kbps Completed 21:43, 6 February 2024 2.0 s
WebM 360P 288 kbps Completed 03:51, 2 December 2023 1.0 s
Streaming 144p (MJPEG) 766 kbps Completed 13:20, 17 November 2023 1.0 s

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