File:Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s007.ogv

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Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s007.ogv ‎(Ogg Theora video file, length 10 s, 222 × 240 pixels, 270 kbps, file size: 331 KB)

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English: PIP3 production induced filopodia and lamellipodia, and endosomes labeled with AktPH-mCh. Time-lapse images of a growth cone of a stage 2 mouse hippocampal neuron expressing AktPH-mCh and the PI3K photoswitch (plasmid #3). AktPH-mCh fluorescence images were acquired every 10 s. The red circled area was continuously photoactivated (405-nm laser at 10% of maximal power, 0.1 Hz for 20 min) from 0 min. Photoactivation of the PI3K photoswitch converted quiescent neurite to motile one with highly active filopodia and lamellipodia. It also significantly increased the area of the growth cone and the emergence of AktPH-labeled vesicles. Selected images from this movie are shown in Fig. 8C,D.
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Source Movie S5 from Kakumoto T, Nakata T (2013). "Optogenetic Control of PIP3: PIP3 Is Sufficient to Induce the Actin-Based Active Part of Growth Cones and Is Regulated via Endocytosis". PLOS ONE. DOI:10.1371/journal.pone.0070861. PMC: 3737352.
Author Kakumoto T, Nakata T
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Date/TimeThumbnailDimensionsUserComment
current06:00, 18 August 201310 s, 222 × 240 (331 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 134 kbps Completed 02:59, 6 September 2018 5.0 s
Streaming 240p (VP9) 135 kbps Completed 21:43, 6 February 2024 1.0 s
WebM 360P 154 kbps Completed 03:51, 2 December 2023 1.0 s
Streaming 144p (MJPEG) 882 kbps Completed 13:20, 17 November 2023 1.0 s

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