File:Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s006.ogv

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Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s006.ogv ‎(Ogg Theora video file, length 9.1 s, 300 × 218 pixels, 1.2 Mbps, file size: 1.3 MB)

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English: Local production of PIP3 by activation of the PI3K photoswitch. Time-lapse images of a stage 2 mouse hippocampal neuron expressing AktPH-mCh and the PI3K photoswitch (plasmid #3). AktPH-mCh fluorescence images were acquired every 10 s and showed in pseudo-color. The red circled areas were continuously photoactivated (405-nm diode laser at 10% of maximal power, 0.1 Hz for 20 min per growth cone) from 0 min. The photoactivated growth cone was changed at 20 min. Photoactivation of the PI3K photoswitch significantly increased AktPH-mCh fluorescence and the area of the growth cone in a light-dependent manner.
Date
Source Movie S4 from Kakumoto T, Nakata T (2013). "Optogenetic Control of PIP3: PIP3 Is Sufficient to Induce the Actin-Based Active Part of Growth Cones and Is Regulated via Endocytosis". PLOS ONE. DOI:10.1371/journal.pone.0070861. PMC: 3737352.
Author Kakumoto T, Nakata T
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Date/TimeThumbnailDimensionsUserComment
current06:00, 18 August 20139.1 s, 300 × 218 (1.3 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 289 kbps Completed 21:43, 6 February 2024 9.0 s
WebM 360P 504 kbps Completed 03:51, 2 December 2023 1.0 s
Streaming 144p (MJPEG) 1.1 Mbps Completed 13:20, 17 November 2023 1.0 s

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