File:Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s003.ogv

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Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s003.ogv ‎(Ogg Theora video file, length 8.1 s, 150 × 146 pixels, 585 kbps, file size: 582 KB)

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English: AktPH-mCh repeatedly moved to the plasma membrane upon photoactivation of the PI3K photoswitch. Time-lapse images of a HEK293 cell expressing AktPH-mCh and the PI3K photoswitch (plasmid #3). AktPH-mCh fluorescence images were acquired every 10 s. Photoactivation was given to whole cell with one pulse of a 442-nm HeCd laser (3%) at 0 min, 15 min and 30 min. Repeated activation successfully translocated AktPH-mCh to the plasma membrane.
Date
Source Movie S1 from Kakumoto T, Nakata T (2013). "Optogenetic Control of PIP3: PIP3 Is Sufficient to Induce the Actin-Based Active Part of Growth Cones and Is Regulated via Endocytosis". PLOS ONE. DOI:10.1371/journal.pone.0070861. PMC: 3737352.
Author Kakumoto T, Nakata T
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Date/TimeThumbnailDimensionsUserComment
current05:59, 18 August 20138.1 s, 150 × 146 (582 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 270 kbps Completed 21:42, 6 February 2024 4.0 s
WebM 360P 322 kbps Completed 03:51, 2 December 2023 1.0 s
Streaming 144p (MJPEG) 1.12 Mbps Completed 13:20, 17 November 2023 1.0 s

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