File:Mouse embryo Trafficking of KIT+ EMPs.jpg
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DescriptionMouse embryo Trafficking of KIT+ EMPs.jpg | Fig 7 Trafficking of KIT+ EMPs. a Schematic graph for the KitMerCreMer:Rosa26mT/mG mouse model with OH-TAM pulse labeling at E7.5 or E8.5 (red arrows) and subsequent analyses at E10.5 (black arrow). b–d Fluorescent pictures of KIT+ cells in the YS and embryo on E10.5 after pulse labeling at indicated time points (b, c) with corresponding quantifications (d); *** p < 0.001 (Mann–Whitney test); median ± IQR. e Quantification of labeled KIT+ cells/min in an average-sized YS vessel at E10.5; median ± IQR. f Velocity of intravascular non-adhering KIT+ cells in the YS at E10.5; mean ± SD. g Flow cytometry plots of CD45+ gated KIT+ vs. GFP+ cells from pulse-labeled KitMerCreMer:Rosa26mT/mG mice in YS, head and trunk region at E10.5. Plots show an individual representative experiment. At least 3 embryos of minimum 2 independent litters were analyzed per time point. Scale bars are 100 µm (b), 1 mm (c) |
Date | |
Source | https://www.nature.com/articles/s41467-017-02492-2/figures/7 Yolk sac macrophage progenitors traffic to the embryo during defined stages of development. Nat Commun 9, 75 (2018). https://doi.org/10.1038/s41467-017-02492-2 |
Author | Stremmel, C., Schuchert, R., Wagner, F. et al. |
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current | 21:10, 21 April 2024 | 1,358 × 1,888 (1.2 MB) | Rasbak (talk | contribs) | == {{int:filedesc}} == {{Information |description=Fig 7 Trafficking of KIT+ EMPs. a Schematic graph for the Kit<sup>MerCreMer</sup>:Rosa26<sup>mT/mG</sup> mouse model with OH-TAM pulse labeling at E7.5 or E8.5 (red arrows) and subsequent analyses at E10.5 (black arrow). b–d Fluorescent pictures of KIT+ cells in the YS and embryo on E10.5 after pulse labeling at indicated time points (b, c) with corresponding quantifications (d); *** p < 0.001 (Mann–Whitney test); median ± IQR. e Quantificatio... |
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