File:Light-Mediated-Kinetic-Control-Reveals-the-Temporal-Effect-of-the-RafMEKERK-Pathway-in-PC12-Cell-pone.0092917.s013.ogv

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Light-Mediated-Kinetic-Control-Reveals-the-Temporal-Effect-of-the-RafMEKERK-Pathway-in-PC12-Cell-pone.0092917.s013.ogv ‎(Ogg Theora video file, length 25 s, 280 × 200 pixels, 188 kbps, file size: 565 KB)

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English: Light-mediated membrane recruitment and dissociation of Raf1 in NIH3T3 cells co-transfected with CIBN-GFP-CaaX and CRY2PHR-mCherry-Raf1. A Nikon A1 confocal microscope was used for image acquisition. The blue 488-nm laser line (0.5 mW, ∼2×106 W/cm2 at the sample plane with a 60× objective) of the confocal microscope was used as the stimulation light. A complete scanning of each frame (512×512 pixels) took 80 ms and a stimulation was applied every 10 s followed by acquiring the mCherry fluorescence image.
Date
Source Movie S1 from Zhang K, Duan L, Ong Q, Lin Z, Varman P, Sung K, Cui B (2014). "Light-Mediated Kinetic Control Reveals the Temporal Effect of the Raf/MEK/ERK Pathway in PC12 Cell Neurite Outgrowth". PLOS ONE. DOI:10.1371/journal.pone.0092917. PMID 24667437. PMC: 3965503.
Author Zhang K, Duan L, Ong Q, Lin Z, Varman P, Sung K, Cui B
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Date/TimeThumbnailDimensionsUserComment
current15:42, 29 March 201425 s, 280 × 200 (565 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 84 kbps Completed 02:33, 15 January 2024 3.0 s
WebM 360P 78 kbps Completed 20:52, 1 December 2023 2.0 s
Streaming 144p (MJPEG) 255 kbps Completed 20:30, 9 November 2023 1.0 s

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