File:In-vivo-single-molecule-imaging-identifies-altered-dynamics-of-calcium-channels-in-dystrophin-ncomms5974-s5.ogv
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DescriptionIn-vivo-single-molecule-imaging-identifies-altered-dynamics-of-calcium-channels-in-dystrophin-ncomms5974-s5.ogv |
English: Supplementary Movie 4 HILO dCALM imaging of two C. elegans worms expressing UNC-36-split-GFP and microinjected with a high concentration of complementary M3 peptides (left panel) or not microinjected (right panel). The objective lens is moved to focus at the muscle sarcolemma and the angle of the 488 nm excitation laser beam is changed to achieve HILO and low background detection of activated UNC-36-split-GFP on body-wall muscles. Activated and fluorescent UNC-36-split-GFP are only detected in the microinjected worm (left panel). Movie acquired at 80 ms per frame and played back at video rate. |
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Source | Video file from Zhan H, Stanciauskas R, Stigloher C, Dizon K, Jospin M, Bessereau J, Pinaud F (2014). "In vivo single-molecule imaging identifies altered dynamics of calcium channels in dystrophin-mutant C. elegans". Nature Communications. DOI:10.1038/ncomms5974. PMID 25232639. PMC: 4199201. | ||
Author | Zhan H, Stanciauskas R, Stigloher C, Dizon K, Jospin M, Bessereau J, Pinaud F | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 09:16, 2 November 2016 | 3.9 s, 1,024 × 512 (6.83 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 4 |
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Author | Zhan H, Stanciauskas R, Stigloher C, Dizon K, Jospin M, Bessereau J, Pinaud F |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | HILO dCALM imaging of two C. elegans worms expressing UNC-36-split-GFP and microinjected with a high concentration of complementary M3 peptides (left panel) or not microinjected (right panel). The objective lens is moved to focus at the muscle sarcolemma and the angle of the 488 nm excitation laser beam is changed to achieve HILO and low background detection of activated UNC-36-split-GFP on body-wall muscles. Activated and fluorescent UNC-36-split-GFP are only detected in the microinjected worm (left panel). Movie acquired at 80 ms per frame and played back at video rate. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014-09-18 |