File:Fluorescent-Protein-Stabilization-and-High-Resolution-Imaging-of-Cleared-Intact-Mouse-Brains-pone.0124650.s007.ogv

English: Coronal maximum projections showing neurons monosynaptically connected to RABV ΔG-EGFP (EnvA) infected source cells in EC. Datasets recorded from same brain as for Fig 4, but after 64 days in clearing solution. Horizontal datasets recorded from the right and left hemisphere were corrected for signal attenuation and aligned using the “TransformJ Rotate” plugin of ImageJ (see Materials and Methods section), followed by stitching of both datasets. From coronal re-slices (downsampled to 6.45 μm cubic voxel size), a sequence of maximum projections (25.8 μm z depth each) was generated from both the green and red fluorescence channel. Label (top left) indicates z position of re-slice related to first image. Red arrow indicates virus injection site and direction. Note the strong red autofluorescence at the periphery of the brain.