File:Filamin-and-Phospholipase-C--Are-Required-for-Calcium-Signaling-in-the-Caenorhabditis-elegans-pgen.1003510.s010.ogv

English: FLN-1 is necessary to trigger calcium signaling. A representative fln-1(tm545) spermathecal transit imaged using xbIs1101[fln-1p::GCaMP]. Oocyte entry occurs from the right at 30 seconds. The sp-ut valve is not constricted despite a pulse of calcium during entry (1 minute) and it appears to dilate, but the oocyte does not exit. During the first ten minutes there is limited calcium release in the distal spermatheca and little constriction. After the first ten minutes stronger calcium pulses commence, and the oocyte moves slightly towards the sp-ut valve wedging it open. The movie is shown twenty times faster than real-time with a lookup table to visualize low intensities (cool colors represent low intensity). Time is shown as minutes∶seconds and begins 30 seconds before oocyte entry. The entire region shown (100 µm×50 µm) was used for the mean fluorescence intensity measurements in Figure 6A.