File:Explosive-cell-lysis-as-a-mechanism-for-the-biogenesis-of-bacterial-membrane-vesicles-and-biofilms-ncomms11220-s12.ogv

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English: Supplementary Movie 10 MVs are formed through curling of membrane fragments produced by explosive cell lysis. A P. aeruginosa PAK biofilm cultured on media containing the lipid stain FM1-43FX was imaged using f3D-SIM (DeltaVision OMX Blaze). A single rod cell transitions to a round-shaped cell and explodes (left panel). A magnified view of the boxed area (right panel) shows the release of shattered membrane fragments which curl and vesicularise into MVs. These newly formed MVs then come to rest on the surface of a neighboring cell. Time is shown in bottom right corner in seconds and milliseconds.
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Source Video file from Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C (2016). "Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms". Nature Communications. DOI:10.1038/ncomms11220. PMID 27075392. PMC: 4834629.
Author Turnbull L, Toyofuku M, Hynen A, Kurosawa M, Pessi G, Petty N, Osvath S, Cárcamo-Oyarce G, Gloag E, Shimoni R, Omasits U, Ito S, Yap X, Monahan L, Cavaliere R, Ahrens C, Charles I, Nomura N, Eberl L, Whitchurch C
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current08:02, 28 October 20164.0 s, 1,010 × 480 (110 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 67 kbps Completed 07:58, 26 August 2018 1.0 s
Streaming 480p (VP9) 68 kbps Completed 03:19, 7 February 2024 1.0 s
VP9 360P 45 kbps Completed 07:58, 26 August 2018 1.0 s
Streaming 360p (VP9) 45 kbps Completed 02:24, 14 March 2024 1.0 s
VP9 240P 29 kbps Completed 07:58, 26 August 2018 2.0 s
Streaming 240p (VP9) 30 kbps Completed 03:52, 21 December 2023 1.0 s
WebM 360P 220 kbps Completed 08:02, 28 October 2016 1.0 s
Streaming 144p (MJPEG) 70 kbps Completed 17:14, 10 November 2023 1.0 s

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