File:Engineering-of-In-Vitro-3D-Capillary-Beds-by-Self-Directed-Angiogenic-Sprouting-pone.0050582.s005.ogv
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Engineering-of-In-Vitro-3D-Capillary-Beds-by-Self-Directed-Angiogenic-Sprouting-pone.0050582.s005.ogv (Ogg Theora video file, length 20 s, 492 × 374 pixels, 481 kbps, file size: 1.15 MB)
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[edit]DescriptionEngineering-of-In-Vitro-3D-Capillary-Beds-by-Self-Directed-Angiogenic-Sprouting-pone.0050582.s005.ogv |
English: Time-lapse imaging of flow through 3D capillary bed. The flow of fluorescent 1 µm diameter beads through the 3D capillary bed was imaged using time-lapse fluorescence microscopy at 10× magnification. After imaging, devices were fixed and immunofluorescently labeled to detect vascular networks: VE-cadherin on cell membranes (anti-VE-cadherin antibody, green), and cell nuclei (DAPI, blue) (actin staining in the red channel is not shown here for image clarity). Images were taken at 500 ms intervals, and the video is playing at 6 frames/s. Scale bar, 100 µm. |
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Source | Video S2 from Chan J, Zervantonakis I, Rimchala T, Polacheck W, Whisler J, Kamm R (2012). "Engineering of In Vitro 3D Capillary Beds by Self-Directed Angiogenic Sprouting". PLOS ONE. DOI:10.1371/journal.pone.0050582. PMID 23226527. PMC: 3514279. | ||
Author | Chan J, Zervantonakis I, Rimchala T, Polacheck W, Whisler J, Kamm R | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:37, 15 December 2012 | 20 s, 492 × 374 (1.15 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Chan J, Zervantonakis I, Rimchala T, Polacheck W, Whisler J, Kamm R |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |