File:Dual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv001.ogv
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Dual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv001.ogv (Ogg Theora video file, length 19 s, 788 × 271 pixels, 71 kbps, file size: 167 KB)
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DescriptionDual-Role-of-Topoisomerase-II-in-Centromere-Resolution-and-Aurora-B-Activity-pbio.0060207.sv001.ogv |
English: Time-Lapse Confocal Microscopy of S2 Cells Stably Expressing CID-GFP and RFP-H2B Z-stacks were acquired every 30 s (time is shown in seconds). Each Z-stack is 10 μm and composed of ten optical sections. Anaphase onset corresponds to time 0 s. Merge color images of RFP-H2B (red) and CID-GFP (green) channels are shown on the left. The CID-GFP (white) channel is shown alone on the right. The two CID-GFP pairs of each centromere were identified and labeled in every layer composing the Z-stacks at the different time points. The mean distance between CID-GFP dots is approximately 1 μm. Tracking of kinetochore pairs was performed using a plug-in for the Image J software. The video of each CID-GFP pair is labeled with the same color. |
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Source | Video S1 from Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C (2008). "Dual Role of Topoisomerase II in Centromere Resolution and Aurora B Activity". PLOS Biology. DOI:10.1371/journal.pbio.0060207. PMID 18752348. PMC: 2525683. | ||
Author | Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 23:23, 30 October 2012 | 19 s, 788 × 271 (167 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Time-Lapse Confocal Microscopy of S2 Cells Stably Expressing CID-GFP and RFP-H2B |
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Author | Coelho P, Queiroz-Machado J, Carmo A, Moutinho-Pereira S, Maiato H, Sunkel C |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Z-stacks were acquired every 30 s (time is shown in seconds). Each Z-stack is 10 ?m and composed of ten optical sections. Anaphase onset corresponds to time 0 s. Merge color images of RFP-H2B (red) and CID-GFP (green) channels are shown on the left. The CID-GFP (white) channel is shown alone on the right. The two CID-GFP pairs of each centromere were identified and labeled in every layer composing the Z-stacks at the different time points. The mean distance between CID-GFP dots is approximately 1 ?m. Tracking of kinetochore pairs was performed using a plug-in for the Image J software. The video of each CID-GFP pair is labeled with the same color. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-08 |
Categories:
- Videos of 2008
- Cell cycle proteins
- Videos of cultured cells of Drosophila melanogaster
- Centromere
- Non-histone chromosomal proteins
- Chromosome segregation
- Type II DNA topoisomerases
- Videos of Drosophila melanogaster proteins
- Enzyme activation
- Schneider 2 cells
- Kinetochores
- Videos of microtubules of the spindle apparatus
- Protein-serine-threonine kinases
- RNA interference
- Sister chromatid exchange
- Topoisomerase II inhibitors
- Aurora kinase B