File:Directing-Astroglia-from-the-Cerebral-Cortex-into-Subtype-Specific-Functional-Neurons-pbio.1000373.s008.ogv

English: Direct visualisation of reprogramming of fate-mapped astroglia into neurons. Temporal sequence of reprogramming by Neurog2 of a fate-mapped astrocyte derived from GLAST::CreERT2/Z/EG mice following recombination in vivo between P2 and P7. The video provides high magnification views of the sequence shown in Video S1. The video starts with a GFP-fluorescence and bright field image at the beginning of the time-lapse experiment. The GFP-positive cell is marked with a red arrow throughout the sequence. The cell is subsequently followed using bright field images. At the point when DsRed fluorescence is detectable, a GFP fluorescence image is shown to demonstrate the co-expression of DsRed and GFP in the same cell. Subsequently DsRed fluorescence images (grey scale) monitor the division of the transduced cell and the gradual metamorphosis of the two daughter cells into neurons. At the end of the imaging sequence a GFP fluorescence image is shown to confirm that the two neurons are reporter-positive. Time is indicated in each image.