File:Direct-Induction-of-Chondrogenic-Cells-from-Human-Dermal-Fibroblast-Culture-by-Defined-Factors-pone.0077365.s010.ogv

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Direct-Induction-of-Chondrogenic-Cells-from-Human-Dermal-Fibroblast-Culture-by-Defined-Factors-pone.0077365.s010.ogv(Ogg Theora video file, length 21 s, 475 × 475 pixels, 1.65 Mbps, file size: 4.14 MB)

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English: Time-lapse images taken during the induction of iChon cells. HDFs were transduced with the lentiviral COL11A2 reporter vector, nucleofected with Slc7a1, and transduced with retroviral c-MYC, KLF4 and SOX9 vectors. Cells were replated onto a well of a 6 well plate immediately after completion of the retroviral transduction. The well was cultured in the absence of puromycin and subjected to time-lapse GFP observation using the Biostation CT program (Nikon). Phase (Movie S1) and GFP (Movie S2) images were captured every 8 h for 14 consecutive days. Each image is shown for 0.5 sec, thus 8 h corresponds to 0.5 sec.
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Source Movie S1 from Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N (2013). "Direct Induction of Chondrogenic Cells from Human Dermal Fibroblast Culture by Defined Factors". PLOS ONE. DOI:10.1371/journal.pone.0077365. PMID 24146984. PMC: 3797820.
Author Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current08:51, 23 October 201321 s, 475 × 475 (4.14 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 469 kbps Completed 11:30, 24 August 2018 7.0 s
Streaming 360p (VP9) 469 kbps Completed 20:43, 6 February 2024 1.0 s
VP9 240P 207 kbps Completed 11:30, 24 August 2018 5.0 s
Streaming 240p (VP9) 207 kbps Completed 22:35, 16 December 2023 1.0 s
WebM 360P 515 kbps Completed 08:52, 23 October 2013 8.0 s
Streaming 144p (MJPEG) 153 kbps Completed 09:46, 3 November 2023 1.0 s

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