File:Direct-Induction-of-Chondrogenic-Cells-from-Human-Dermal-Fibroblast-Culture-by-Defined-Factors-pone.0077365.s010.ogv
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Direct-Induction-of-Chondrogenic-Cells-from-Human-Dermal-Fibroblast-Culture-by-Defined-Factors-pone.0077365.s010.ogv (Ogg Theora video file, length 21 s, 475 × 475 pixels, 1.65 Mbps, file size: 4.14 MB)
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DescriptionDirect-Induction-of-Chondrogenic-Cells-from-Human-Dermal-Fibroblast-Culture-by-Defined-Factors-pone.0077365.s010.ogv |
English: Time-lapse images taken during the induction of iChon cells. HDFs were transduced with the lentiviral COL11A2 reporter vector, nucleofected with Slc7a1, and transduced with retroviral c-MYC, KLF4 and SOX9 vectors. Cells were replated onto a well of a 6 well plate immediately after completion of the retroviral transduction. The well was cultured in the absence of puromycin and subjected to time-lapse GFP observation using the Biostation CT program (Nikon). Phase (Movie S1) and GFP (Movie S2) images were captured every 8 h for 14 consecutive days. Each image is shown for 0.5 sec, thus 8 h corresponds to 0.5 sec. |
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Source | Movie S1 from Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N (2013). "Direct Induction of Chondrogenic Cells from Human Dermal Fibroblast Culture by Defined Factors". PLOS ONE. DOI:10.1371/journal.pone.0077365. PMID 24146984. PMC: 3797820. | ||
Author | Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 08:51, 23 October 2013 | 21 s, 475 × 475 (4.14 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Time-lapse images taken during the induction of iChon cells. HDFs were transduced with the lentiviral COL11A2 reporter vector, nucleofected with Slc7a1, and transduced with retroviral c-MYC, KLF4 and SOX9 vectors. Cells were replated onto a well of a 6 well plate immediately after completion of the retroviral transduction. The well was cultured in the absence of puromycin and subjected to time-lapse GFP observation using the Biostation CT program (Nikon). Phase (Movie S1) and GFP (Movie S2) images were captured every 8 h for 14 consecutive days. Each image is shown for 0.5 sec, thus 8 h corresponds to 0.5 sec. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |