File:Differential-pH-dependent-cellular-uptake-pathways-among-foamy-viruses-elucidated-using-dual-1742-4690-9-71-S6.ogv

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English: Movie. FV uptake and trafficking in Bafilomycin A1 treated cells. Hela cells pretreated with 60 nm BAF were incubated with double-tagged PFV Gag-eGFP (non-spiked) and either PE Ch, SE Ch or SE Ch iCS containing viral particles at low temperature (~10°C) in the presence of the drug. After warming the cell to 37°C, z-stacks with 13 planes were recorded every 15 minutes for a total of 12 h using live-cell wide-field microscopy. The video shows the DIC channel (upper channels), the green Gag-eGFP channel (middle channels) shown in gray and a colored overlay of both channels with Gag-eGFP in green and Ch-Env in red (“merge”, lower channels). The individual fluorescence channels and the “merge” channel are displayed as maximum intensity z-projection of each z-stack. scale bar: 10 μm.
Date
Source Stirnnagel K, Schupp D, Dupont A, Kudryavtsev V, Reh J, Müllers E, Lamb D, Lindemann D (2012). "Differential pH-dependent cellular uptake pathways among foamy viruses elucidated using dual-colored fluorescent particles". Retrovirology. DOI:10.1186/1742-4690-9-71. PMID 22935135. PMC: 3495412.
Author Stirnnagel K, Schupp D, Dupont A, Kudryavtsev V, Reh J, Müllers E, Lamb D, Lindemann D
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current09:31, 20 November 20126.9 s, 1,437 × 1,356 (8.71 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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