File:Differential-pH-dependent-cellular-uptake-pathways-among-foamy-viruses-elucidated-using-dual-1742-4690-9-71-S4.ogv

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Original file ‎(Ogg Theora video file, length 3.5 s, 972 × 1,002 pixels, 7.28 Mbps, file size: 3.08 MB)

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English: Movie. Gag tethering to host cell chromatin during mitosis. HeLa cells were incubated with double-tagged PFV Gag-eGFP (non-spiked) and either PE Ch or PE Ch iCS containing viral particles at low temperature (~10°C). After warming the cells to 37°C, z-stacks with 12 planes were recorded every 15 minutes for a total of 6 h using wide-field live-cell microscopy. The video shows the DIC channel (upper panels), the Gag-eGFP channel (middle panels) displayed in gray and an overlay of these two channels (lower panels) for the PE Ch containing particles (right channels) and PE Ch iCS containing particles (right channels). The individual fluorescence channels and the “merge” channel are displayed as maximum intensity z-projection of each z-stack. The DIC channel was adjusted according to brightness and contrast using ImageJ PlugIns.
Date
Source Stirnnagel K, Schupp D, Dupont A, Kudryavtsev V, Reh J, Müllers E, Lamb D, Lindemann D (2012). "Differential pH-dependent cellular uptake pathways among foamy viruses elucidated using dual-colored fluorescent particles". Retrovirology. DOI:10.1186/1742-4690-9-71. PMID 22935135. PMC: 3495412.
Author Stirnnagel K, Schupp D, Dupont A, Kudryavtsev V, Reh J, Müllers E, Lamb D, Lindemann D
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current09:30, 20 November 20123.5 s, 972 × 1,002 (3.08 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 720P 1.65 Mbps Completed 06:44, 24 August 2018 5.0 s
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