File:Differential-pH-dependent-cellular-uptake-pathways-among-foamy-viruses-elucidated-using-dual-1742-4690-9-71-S1.ogv

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Original file ‎(Ogg Theora video file, length 6.2 s, 1,672 × 1,932 pixels, 9.81 Mbps, file size: 7.25 MB)

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English: Movie. Comparison of syncytia formation in PFV or SFVmac infected cells. HeLa cells were incubated with double-tagged PFV Gag-eGFP (non-spiked) and either PE Ch or SE Ch containing viral particles at low temperature (~10°C). After warming the cells to 37°C, z-stacks with 13 planes were recorded every 15 minutes for a total of 7.5 h using wide-field live-cell microscopy. In the upper part of the video, the DIC images for the PE Ch containing particles (left panels) or SE Ch containing particles (right panels) are shown. In the lower part of the movie, the corresponding fluorescence overlay images of the red (Ch-Env) and the green (Gag-eGFP) channels are depicted. The overlay images are displayed as maximum intensity z-projection of each z-stack. The DIC channel was adjusted according to brightness and contrast using ImageJ PlugIns. Scale bar: 20 μm.
Date
Source Stirnnagel K, Schupp D, Dupont A, Kudryavtsev V, Reh J, Müllers E, Lamb D, Lindemann D (2012). "Differential pH-dependent cellular uptake pathways among foamy viruses elucidated using dual-colored fluorescent particles". Retrovirology. DOI:10.1186/1742-4690-9-71. PMID 22935135. PMC: 3495412.
Author Stirnnagel K, Schupp D, Dupont A, Kudryavtsev V, Reh J, Müllers E, Lamb D, Lindemann D
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current09:30, 20 November 20126.2 s, 1,672 × 1,932 (7.25 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 1440P 5.7 Mbps Completed 06:43, 24 August 2018 14 s
Streaming 1440p (VP9) 5.7 Mbps Completed 12:53, 13 January 2024 1.0 s
VP9 1080P 2.73 Mbps Completed 06:43, 24 August 2018 10 s
Streaming 1080p (VP9) 2.73 Mbps Completed 05:53, 6 February 2024 1.0 s
VP9 720P 1.37 Mbps Completed 06:43, 24 August 2018 7.0 s
Streaming 720p (VP9) 1.37 Mbps Completed 21:54, 13 March 2024 2.0 s
VP9 480P 611 kbps Completed 06:43, 24 August 2018 7.0 s
Streaming 480p (VP9) 611 kbps Completed 13:22, 2 February 2024 1.0 s
VP9 360P 283 kbps Completed 06:43, 24 August 2018 5.0 s
Streaming 360p (VP9) 284 kbps Completed 19:11, 6 February 2024 1.0 s
VP9 240P 107 kbps Completed 06:43, 24 August 2018 2.0 s
Streaming 240p (VP9) 108 kbps Completed 21:23, 16 December 2023 1.0 s
WebM 360P 510 kbps Completed 10:08, 20 November 2012 4.0 s
Streaming 144p (MJPEG) 226 kbps Completed 08:26, 3 November 2023 1.0 s

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