File:Detachment-of-Breast-Tumor-Cells-Induces-Rapid-Secretion-of-Exosomes-Which-Subsequently-Mediate-pone.0024234.s003.ogv
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Detachment-of-Breast-Tumor-Cells-Induces-Rapid-Secretion-of-Exosomes-Which-Subsequently-Mediate-pone.0024234.s003.ogv (Ogg Theora video file, length 7.6 s, 480 × 480 pixels, 1.57 Mbps, file size: 1.43 MB)
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DescriptionDetachment-of-Breast-Tumor-Cells-Induces-Rapid-Secretion-of-Exosomes-Which-Subsequently-Mediate-pone.0024234.s003.ogv |
English: BT-CD63 cells were plated on MatTek glass dishes for 24 hours. Prior to live-cell imaging cells were washed 2 times in PBS. Cells were then treated with EDTA (2 mM) and the trafficking and release of GFP-labeled exosomes monitored by time-lapse imaging for up to 10 minutes. GFP-labeled exosomes shoot out as the cells retract and by the end of the movie, most of the GFP labeled particles have moved outside. |
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Source | Movie S2 from Koumangoye R, Sakwe A, Goodwin J, Patel T, Ochieng J (2011). "Detachment of Breast Tumor Cells Induces Rapid Secretion of Exosomes Which Subsequently Mediate Cellular Adhesion and Spreading". PLOS ONE. DOI:10.1371/journal.pone.0024234. PMID 21915303. PMC: 3167827. | ||
Author | Koumangoye R, Sakwe A, Goodwin J, Patel T, Ochieng J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 16:43, 14 November 2012 | 7.6 s, 480 × 480 (1.43 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Koumangoye R, Sakwe A, Goodwin J, Patel T, Ochieng J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | BT-CD63 cells were plated on MatTek glass dishes for 24 hours. Prior to live-cell imaging cells were washed 2 times in PBS. Cells were then treated with EDTA (2 mM) and the trafficking and release of GFP-labeled exosomes monitored by time-lapse imaging for up to 10 minutes. GFP-labeled exosomes shoot out as the cells retract and by the end of the movie, most of the GFP labeled particles have moved outside. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |