File:Cellular-Contractility-Requires-Ubiquitin-Mediated-Proteolysis-pone.0006155.s009.ogv

English: Nocodazole induced contractions depend on ubiquitination. A31N-wt and A31N-ts20 cells were subjected to nocodazole at the permissive and restrictive temperatures. A, A31N-wt control cells undergo normal mitosis at 39.5°. B, An A31N-wt cell grown at 39.5° was treated with nocodazole and contracted upon mitotic entry. C, An A31N-ts20 cell grown at 34°, was treated with nocodazole. The cell contracted for 7 hours before it flattened. D, An A31N-ts20 cell was grown at 39.5° in the presence of nocodazole for 13 hours before it entered mitosis (788 min). During the next 14 hours the cell remained arrested, did not contracted and did not flatten.