File:Axonal-BACE1-dynamics-and-targeting-in-hippocampal-neurons-a-role-for-Rab11-GTPase-1750-1326-9-1-S4.ogv

English: Dynamic Colocalization BACE1with Rab11b in Dendrites and Axons. Hippocampal neurons (DIV14) cotransfected with BACE1-YFP and mCherry-Rab11b were observed by live-cell imaging. Dual-color time-lapse images of a dendritic branch and an axon were acquired using the Dual View imaging system at a rate of 1 frame/sec for 3 min, with an exposure time of 300 ms, and displayed at 7 frames/sec. In the dendrite (top), bidirectional movement of a carrier containing BACE1 and Rab11b, emerging from a larger stationary structure, which also contains both the proteins, is indicated by a pink arrowhead; a rapidly moving vesicle containing both BACE1 and Rab11 is indicated by a blue arrowhead. In the axon (bottom), blue and pink arrowheads indicate rapidly moving carriers that contain both the proteins. The anterograde direction of movement is toward the right. Scale bar 20 μm. Representative kymographs are shown in Figure 5C.