File:A-Novel-Reporter-Rat-Strain-That-Conditionally-Expresses-the-Bright-Red-Fluorescent-Protein-tdTomato-pone.0155687.s005.ogv

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A-Novel-Reporter-Rat-Strain-That-Conditionally-Expresses-the-Bright-Red-Fluorescent-Protein-tdTomato-pone.0155687.s005.ogv(Ogg Theora video file, length 6.0 s, 320 × 222 pixels, 4.07 Mbps, file size: 2.91 MB)

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English: Acquired coronal images were reconstructed in 3D. Related to Fig 3. Scale bar: 50 μm.

Fig 3 Three-dimensional imaging of hippocampal CA1 after tissue clearing.

(A) Three-dimensional reconstruction of the CA1 region of the hippocampus, which was treated with CUBIC reagent 1 for 2 days and imaged under two-photon microscopy. The laminar structure of pyramidal cells was obvious. Axons and dendrites were brightly labeled by tdTomato and easily traced throughout the tissue.

(B) X–Y, X–Z, and Y–Z planar sections of the image shown in (A).
Date
Source S1 Video from Igarashi H, Koizumi K, Kaneko R, Ikeda K, Egawa R, Yanagawa Y, Muramatsu S, Onimaru H, Ishizuka T, Yawo H (2016). "A Novel Reporter Rat Strain That Conditionally Expresses the Bright Red Fluorescent Protein tdTomato". PLOS ONE. DOI:10.1371/journal.pone.0155687. PMID 27195805. PMC: 4873025.
Author Igarashi H, Koizumi K, Kaneko R, Ikeda K, Egawa R, Yanagawa Y, Muramatsu S, Onimaru H, Ishizuka T, Yawo H
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Date/TimeThumbnailDimensionsUserComment
current03:46, 13 October 20166.0 s, 320 × 222 (2.91 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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