File:A-Novel-3D-Fibril-Force-Assay-Implicates-Src-in-Tumor-Cell-Force-Generation-in-Collagen-Networks-pone.0058138.s010.ogv
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DescriptionA-Novel-3D-Fibril-Force-Assay-Implicates-Src-in-Tumor-Cell-Force-Generation-in-Collagen-Networks-pone.0058138.s010.ogv |
English: Rotating 3D view of the cell from Fig. 3. A. Cyan: collagen fibrils. Yellow: Actin labled by Alexa-Fluor 568 Phalloidin. Magenta: eGFP-ca-Src. In both cell types, F-actin is localized primarily at the cell periphery. See Fig. S2 for gallery views of individual z-slices, and Movies S8 and S9 for rotating 3D views. Cells were fixed after 6 hours in the 3D collagen network. Use of a higher-N.A. objective in the live-cell allows for better resolution of both Src position (see methods). The actin channel is turned off briefly at the end to maximize visibility. |
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Source | Movie S8 from Polackwich R, Koch D, Arevalo R, Miermont A, Jee K, Lazar J, Urbach J, Mueller S, McAllister R (2013). "A Novel 3D Fibril Force Assay Implicates Src in Tumor Cell Force Generation in Collagen Networks". PLOS ONE. DOI:10.1371/journal.pone.0058138. PMID 23536784. PMC: 3594227. | ||
Author | Polackwich R, Koch D, Arevalo R, Miermont A, Jee K, Lazar J, Urbach J, Mueller S, McAllister R | ||
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current | 21:46, 3 June 2013 | 20 s, 1,122 × 764 (15.29 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Polackwich R, Koch D, Arevalo R, Miermont A, Jee K, Lazar J, Urbach J, Mueller S, McAllister R |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | Rotating 3D view of the cell from Fig. 3. A. Cyan: collagen fibrils. Yellow: Actin labled by Alexa-Fluor 568 Phalloidin. Magenta: eGFP-ca-Src. In both cell types, F-actin is localized primarily at the cell periphery. See Fig. S2 for gallery views of individual z-slices, and Movies S8 and S9 for rotating 3D views. Cells were fixed after 6 hours in the 3D collagen network. Use of a higher-N.A. objective in the live-cell allows for better resolution of both Src position (see methods). The actin channel is turned off briefly at the end to maximize visibility. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |