File:A-Bispecific-Antibody-Promotes-Aggregation-of-Ricin-Toxin-on-Cell-Surfaces-and-Alters-Dynamics-of-pone.0156893.s007.ogv

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A-Bispecific-Antibody-Promotes-Aggregation-of-Ricin-Toxin-on-Cell-Surfaces-and-Alters-Dynamics-of-pone.0156893.s007.ogv(Ogg Theora video file, length 30 s, 512 × 512 pixels, 180 kbps, file size: 660 KB)

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English: Live cell imaging of ricin-FITC and TGN. Real time imaging of A549 cells visualizing ricin-FITC in green and TGN in red labeled with a transient transfection as described in Material and Methods. The total fluorescence for the TGN marker is always overwhelming compared to ricin-FITC and therefore the absolute co-localization between ricin-FITC and the TGN is not apparent in the raw images prior to processing. Real time images were processed prior to quantification as described in Materials and Methods to determine co-localization values, which are reported in Fig 4B. Total length of the time lapse is 120 min.
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Source S1 Movie from Herrera C, Klokk T, Cole R, Sandvig K, Mantis N (2016). "A Bispecific Antibody Promotes Aggregation of Ricin Toxin on Cell Surfaces and Alters Dynamics of Toxin Internalization and Trafficking". PLOS ONE. DOI:10.1371/journal.pone.0156893. PMID 27300140. PMC: 4907443.
Author Herrera C, Klokk T, Cole R, Sandvig K, Mantis N
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Date/TimeThumbnailDimensionsUserComment
current02:16, 13 October 201630 s, 512 × 512 (660 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 56 kbps Completed 15:44, 14 February 2023 3.0 s
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WebM 360P 107 kbps Completed 15:44, 14 February 2023 1.0 s
Streaming 144p (MJPEG) 35 kbps Completed 06:36, 29 October 2023 1.0 s

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