File:Reelin-induced radial glial phenotype is dependent on gamma-secretase activity.jpg
From Wikimedia Commons, the free media repository
Jump to navigation
Jump to search
Size of this preview: 495 × 600 pixels. Other resolutions: 198 × 240 pixels | 396 × 480 pixels | 634 × 768 pixels | 1,200 × 1,454 pixels.
Original file (1,200 × 1,454 pixels, file size: 622 KB, MIME type: image/jpeg)
File information
Structured data
Captions
DescriptionReelin-induced radial glial phenotype is dependent on gamma-secretase activity.jpg |
English: Reelin-induced radial glial phenotype is dependent on γ-secretase activity. HNPCs were deprived of FGF and EGF for 24 hours prior to their transfection or treatment. a, g, HNPCs were transfected with empty vector or d, j NICD for 24 hours before fixation. The percentage of the BLBP-positive radial glia increased from 2.3% in the empty vector transfected cells to 6.2% in the NICD transfected cells, *p-value = 0.05. b, h, HNPCs were treated with partially purified control or c, i, partially purified Reelin for 24 hours before fixation. The percentage of the BLBP-positive radial glia increased from 3.4 % in the control-treated cells to 9.5% in the Reelin-treated cells, **p-value = 0.05. e, k, f, l, 10 μM of L-685,458 was used to inhibit the activity of γ-secretase for 24 hours in the control and the Reelin treated HNPCs. γ-secretase inhibition in the Reelin treated cells abolished Reelin's effect by reducing the percentage of the BLBP-positive radial glia back to 1.6 %, ***p-value = 0.05. The results represent the standard error of the mean ± SEM of at least three independent experiments. One-way ANOVA with Tukey's post hoc test for individual treatment differences was used for statistical analysis. Values that are significantly different from each other according to Tukey's test are indicated by asterisks. Scale bar for a-f is 100 μm and for g-l is 50 μm.
Keilani and Sugaya BMC Developmental Biology 2008 8:69 doi:10.1186/1471-213X-8-69 |
Date | |
Source | http://www.biomedcentral.com/1471-213X/8/69 |
Author | Serene Keilani and Kiminobu Sugaya |
Permission (Reusing this file) |
© 2008 Keilani and Sugaya; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Other versions | Derivative works of this file: Reelin induces GFAP and FABP7 via Notch1.jpgno |
This file is licensed under the Creative Commons Attribution 2.0 Generic license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 20:14, 4 August 2008 | 1,200 × 1,454 (622 KB) | CopperKettle (talk | contribs) | {{Information |Description={{en|1=Reelin-induced radial glial phenotype is dependent on γ-secretase activity. HNPCs were deprived of FGF and EGF for 24 hours prior to their transfection or treatment. a, g, HNPCs were transfected with empty vector or d, j |
You cannot overwrite this file.
File usage on Commons
The following page uses this file:
Metadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
_error | 0 |
---|