File:Pre-gastrulation-cell-movements-are-impaired-by-ROCK-inhibition-A-D-F-I-Dorsal-views a.jpg

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Description Fig. 1. Pre-gastrulation cell movements are impaired by ROCK inhibition. (A-D,F-I) Dorsal views of control (A-D) and 40 µM ROCK-inhibited (F-I) rabbit embryonic discs taken at the beginning (A,C,F,H) and the end (B,D,G,I) of the timelapse movie (supplementary material Movies 1,2). Asterisks mark embryonic disc borders. (E,J) Paths of the traced cells relative to the posterior gastrula extension (PGE) area (anterior border marked by dashed lines). (K,L) Processional cell movement in control embryo (see the cell marked in red passing between the cells marked in blue and yellow). (M,N) Cell intercalation in treated embryo (see the cell marked in red squeezed between the cells marked in green and orange in M,N). (O,P) High magnification of DIC recordings, showing cell form and protrusions defined by the quotient of the long cell axis (b) divided by the short cell axis (a) in control (O) and treated (P) embryo. Square bracket in O marks metaphase plate. (Q,R) Quantitative analysis of the orientation of the longest cell protrusions related to the anteriorposterior (AP) axis (0°±180°) in control (Q) and in treated (R) embryos using measurements of the angles between the longest cell axis and the AP axis. Radial graduation shows the number of cells that fall within a specific angular region and orbital graduation shows the angles. Scale bars: 250 µm in A for A,B; 50 µm in C for C-E,H-J; 175 µm in F for F,G; 5 µm in P for K-P.
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Source https://journals.biologists.com/dev/article/142/1/92/46970/Rho-kinase-activity-controls-directional-cell Rho kinase activity controls directional cell movements during primitive streak formation in the rabbit embryo. Development. 2015 Jan 1;142(1):92-8. doi: 10.1242/dev.111583. PMID: 25516971; PMCID: PMC4299133.
Author Stankova V, Tsikolia N, Viebahn C.
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© 2015. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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current13:59, 28 April 2024Thumbnail for version as of 13:59, 28 April 20241,233 × 1,293 (1.14 MB)Rasbak (talk | contribs){{Information |description=Fig. 1. Pre-gastrulation cell movements are impaired by ROCK inhibition. (A-D,F-I) Dorsal views of control (A-D) and 40 µM ROCK-inhibited (F-I) rabbit embryonic discs taken at the beginning (A,C,F,H) and the end (B,D,G,I) of the timelapse movie (supplementary material Movies 1,2). Asterisks mark embryonic disc borders. (E,J) Paths of the traced cells relative to the posterior gastrula extension (PGE) area (anterior border marked by dashed lines). (K,L) Processional...

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