File:PCP4 Western blot with knock-down and control H295R adrenocortical cells.jpg

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English: Western blotting of PCP4 (down, 7.6kDa) and beta-actin (control marker, up, 42kDa) in two samples of H295R human adrenocortical carcinoma cells. Sample number 1 was submitted to PCP4 transient knockdown using a MISSION® esiRNA (SIGMA-ALDRICH), while sample number 2 was transfected with a universal negative-control siRNA. H295R cells were transfected using a Nucleofector-4D (LONZA). All methods were carried out as published by Felizola SJA et al 2014 Journal of Molecular Endocrinology 52(2):159-67. doi: 10.1530/JME-13-0248.[1]
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Source Western blotting of PCP4 and beta-actin (control marker) in two samples of H295R human adrenal cortex carcinoma cell line according to published methods.
Author Herizora
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English: Western blotting of PCP4 (down, 7.6kDa) and beta-actin (control marker, up, 42kDa) in two samples of H295R human adrenal carcinoma cells. Sample number 1 was submitted to PCP4 transient knockdown using a MISSION® esiRNA (SIGMA-ALDRICH), while sample number 2 was transfected with a universal negative-control siRNA. H295R cells were transfected using a Nucleofector-4D (LONZA). PCP4 antibody (rabbit anti-human, polyclonal) was purchased from SIGMA-ALDRICH and used at 1:400 concentration. All methods were carried out as published by Felizola SJA et al 2014 Journal of Molecular Endocrinology 52(2):159-67. doi: 10.1530/JME-13-0248.[1]

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  1. ↑ a b Saulo J.A. Felizola, Yasuhiro Nakamura, Yoshikiyo Ono, Kanako Kitamura, Kumi Kikuchi, Yoshiaki Onodera, Kazue Ise, Kei Takase, Akira Sugawara, Namita Hattangady, William E. Rainey, Fumitoshi Satoh, Hironobu Sasano. (Feb 2014). "PCP4: a regulator of aldosterone synthesis in human adrenocortical tissues.". Journal of Molecular Endocrinology 52 (2): 159-167. DOI:10.1530/JME-13-0248. PMID 24403568.

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current05:33, 17 October 2014Thumbnail for version as of 05:33, 17 October 20141,248 × 1,349 (81 KB)Herizora (talk | contribs){{subst:Upload marker added by en.wp UW}} {{Information |Description = {{en|Western blotting of PCP4 (down, 7.6kDa) and beta-actin (control marker, up, 42kDa) in two samples of H295R human adrenocortical carcinoma cells. Sample number 1 was submitt...

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