File:Laser capture microdissection and four staining methods.jpg

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English: Comparison of four staining methods. Images were taken directly on the PALM Microbeam to demonstrate the staining and image quality available to identify the different cell populations.

a. Hematoxylin. b. Hematoxylin and Eosin. c. 0.1% cresyl violet in H2O. d. 1% cresyl violet in H2O.
For hematoxylin and H&E staining (a, b), sections were rinsed with 70% EtOH and H2O, stained with Mayer's hematoxylin for 15 sec, rinsed with H2O and 70% EtOH and stained with Accustain Eosin Y for 10 sec (for H&E only).
For cresyl violet staining (c, d), sections were rehydrated in decreasing concentrations of ethanol, stained in cresyl violet for 45 sec and dehydrated in increasing concentrations of ethanol. 1% cresyl violet (d) provided the best morphological details and allowed clear distinction between cortical plate and subplate.
e, f. Localization of the subplate at E15. Depth and width of the subplate layer on cresyl violet stained sections (e) were confirmed with immunohistochemistry against Nurr1 (f).
Scalebars: 100 μm. MZ = marginal zone, CP = cortical plate, SP = subplate, IZ = intermediate zone, SVZ = subventricular zone, VZ = ventricular zone.

Mouse.
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Source Wang et al. High quality RNA from multiple brain regions simultaneously acquired by laser capture microdissection, BMC Molecular Biology 2009 10:69 doi:10.1186/1471-2199-10-6
Author Wei-Zhi Wang* , Franziska M Oeschger* , Sheena Lee and Zoltán Molnár
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current20:25, 22 January 2014Thumbnail for version as of 20:25, 22 January 2014900 × 1,203 (373 KB)Staticshakedown (talk | contribs)Reverted to version as of 10:29, 15 August 2009
20:23, 22 January 2014Thumbnail for version as of 20:23, 22 January 2014395 × 645 (110 KB)Cropbot (talk | contribs)upload cropped version, operated by User:Staticshakedown. Summary: cropped
10:29, 15 August 2009Thumbnail for version as of 10:29, 15 August 2009900 × 1,203 (373 KB)CopperKettle (talk | contribs){{Information |Description={{en|1=Comparison of four staining methods. Images were taken directly on the PALM Microbeam to demonstrate the staining and image quality available to identify the different cell populations. a. Hematoxylin. b. Hematoxylin and

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