File:Fmicb-07-02022-g005.jpg

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English: Growth of Pseudochlorella sp. YKT1 cells in M-Allen medium (MA), nitrogen-free MA medium (MA-N) and media derived from acidic hot spring waters supplemented with different nitrogen sources (pH 2.0). Cells cultured in MA (OD750 of 1.0–2.0) were collected by centrifugation, gently resuspended into the respective media to give an OD750 of 1.0 and then cultured for 7 days. (A, B) Photographs of the cultures in a 24-well plate at 7 days after inoculation. (C) OD750 of cultures at 7 days after inoculation. The bar indicates the standard deviation of three independent experiments. Significance was calculated by t-test. ∗Statistically significant differences at P < 0.05; ∗∗statistically significant differences at P < 0.01. (D) Micrographs of cells that were cultured in the respective media for 7 days. Ta: Tamagawa, Tsu: Tsukahara. Scale bar = 5 μm.
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Source Cultivation of Acidophilic Algae Galdieria sulphuraria and Pseudochlorella'¡ sp. YKT1 in Media Derived from Acidic Hot Springs. In: Front. Microbiol. - Volume 7 - 2016. doi:10.3389/fmicb.2016.02022.
Author Shunsuke Hirooka & Shin-ya Miyagishima
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