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DescriptionEaay9634.F1.large.jpg |
English: Characterization of Gemytripvirus fugra1(alias Fusarium graminearum gemytripvirus 1, FgGMTV1, fam. Genomoviridae) infecting F. graminearum strain HB58 and composition of the isolated virus particles.
(A) Colony morphology of virus-carrying field strain HB58 of F. graminearum after 3 days of culture on potato dextrose agar (PDA) in the dark. (B) Viral particles observed under transmission electron microscopy. (C) One percent agarose gel electrophoresis of DNA extracted from virus particles. bp, base pair. (D) Electrophoresis analysis of enzyme-treated nucleic acid samples on 1% agarose gel. The samples were treated with DNase I, exonuclease III, S1 nuclease, and exonuclease I, respectively. (E) SDS–polyacrylamide gel electrophoresis analysis of purified virus particles from strain HB58 showing three protein bands. (F) Southern blot analyses of nucleic acids extracted from viral particles. Genomic DNA extracted from strain HB58 was used as a positive control. The forms of the viral DNA are indicated as OC dsDNA (open circular dsDNA), SC dsDNA (supercoiled dsDNA), and circular ssDNA. Three DNA fragments (probe A, probe B, and probe C, specific for DNA-A, DNA-B, and DNA-C, respectively) were PCR-amplified, labeled with DIG, and used as DNA probes. (G) Genome organization of the three components of FgGMTV1. The single ORF of each DNA component is displayed as a thick arrow. The positions of the potential stem-loop structure, common region major (CR-M) and common region stem-loop (CR-SL) are indicated. Photo credit: Pengfei Li, Institute of Plant Protection, Chinese Academy of Agricultural Sciences. |
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Source | Fig. 1 at https://advances.sciencemag.org/content/6/14/eaay9634 A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions. In: Science Advances Vol. 6, no. 14, eaay9634, doi:10.1126/sciadv.aay9634 | ||
Author | Pengfei Li, Shuangchao Wang, Lihang Zhang, Dewen Qiu, Xueping Zhou, Lihua Guo | ||
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File change date and time | 19:42, 27 March 2020 |
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Date and time of digitizing | 03:41, 28 March 2020 |
Date metadata was last modified | 03:42, 28 March 2020 |
Unique ID of original document | xmp.did:af90bc3c-ad56-1549-b55f-61b0ce8e78e2 |