File:Comparison of Cas9-, D10A-, and H840A-induced repair phenotypes.jpg
From Wikimedia Commons, the free media repository
Jump to navigation
Jump to search
Size of this preview: 469 × 599 pixels. Other resolutions: 188 × 240 pixels | 376 × 480 pixels | 601 × 768 pixels | 801 × 1,024 pixels | 1,603 × 2,048 pixels | 3,334 × 4,259 pixels.
Original file (3,334 × 4,259 pixels, file size: 1.36 MB, MIME type: image/jpeg)
File information
Structured data
Captions
Summary[edit]
DescriptionComparison of Cas9-, D10A-, and H840A-induced repair phenotypes.jpg |
English: "(A) Cas9, D10A, and H840A nucleases expressed under the control of vasa promoter from three equivalent insertions (in X chromosome locus yellow) were used to induce allelic repair in yccw wATG− CR+/yvasaCas9 wATG+ CS1−, yccw wATG− CR+/yvasaD10A wATG+ CS1−, and yccw wATG− CR+/yvasaH840A wATG+ CS1− females. Control animals were yccw wATG− CR+/y+ wATG+ CS1. Left panels show typical repair phenotypes, and diagrams on the right represent cutting or nicking by each different nuclease. (B) Allelic repair was quantified by image analysis using ImageJ. Nickases are more efficient than Cas9 in eliciting HTR, and H840A is more efficient than D10A for repairing the CS1− allele. (C) Quantitative analysis of HTR of the CS1− allele by deep sequencing. Correction percentages after adjustments (see Materials and Methods) from five independent reads were plotted for each genotype (no nuclease control CR+/CS1−, +Cas9, +D10A, and +H840A). Results confirm the trend calculated using pigment and Sanger sequencing analysis: Repair by D10A is significantly higher (41%) than by Cas9 (27%). H840-elicited repair (51%) is significantly more efficient than by D10A. ***P < 0.001 and *P < 0.05. (D) Pie chart representation of deep sequencing analysis. Color coding—pink, donor CR+ alleles; white, intact CS1− alleles; dark purple, NHEJ mutations (centered at cut site); gray, PCR-induced recombination #1 and some asymmetrical HTR and NHEJ events (only for Cas9, D10A, and H840 samples; see fig. S9); light blue sectors, PCR-induced recombination #2; light purple sectors, PCR-induced substitutions. This representation allows global visualization of different categories of events following Cas9-, D10A-, and H840-dependent cleavage in wATG− CR+/y+ wATG+ CS1− individuals. Nickases are more effective at producing HTR than Cas9, with H840 inducing the highest levels of conversion. Cas9 induces high levels of NHEJ events, while D10A and H840 only elicit low levels of NHEJ and leave ~18 to 23% of intact CS1− alleles." |
Date | |
Source | https://www.science.org/doi/10.1126/sciadv.abo0721 |
Author | Authors of the study: Sitara Roy, Sara Sanz Juste, Marketta Sneider Ankush Auradkar, Carissa Klanseck, Zhiqian Li, Alison Henrique, Ferreira Julio, Victor Lopez del Amo, Ethan Bier and Annabel Guichard |
Licensing[edit]
This file is licensed under the Creative Commons Attribution 4.0 International license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 21:52, 27 August 2022 | 3,334 × 4,259 (1.36 MB) | Prototyperspective (talk | contribs) | Uploaded a work by Authors of the study: Sitara Roy, Sara Sanz Juste, Marketta Sneider Ankush Auradkar, Carissa Klanseck, Zhiqian Li, Alison Henrique, Ferreira Julio, Victor Lopez del Amo, Ethan Bier and Annabel Guichard from https://www.science.org/doi/10.1126/sciadv.abo0721 with UploadWizard |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
File usage on other wikis
The following other wikis use this file:
- Usage on en.wikipedia.org
Metadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Short title |
|
---|---|
Date metadata was last modified | 05:34, 24 June 2022 |
File change date and time | 05:34, 24 June 2022 |
Date and time of digitizing | 17:29, 21 June 2022 |
Software used | Adobe Illustrator CC 23.0 (Macintosh) |
Unique ID of original document | uuid:5D20892493BFDB11914A8590D31508C8 |
Structured data
Items portrayed in this file
depicts
21 June 2022
image/jpeg
4e70bee08ac822bdcc10f1b2e26c412eb951bdf4
1,420,916 byte
4,259 pixel
3,334 pixel
Hidden category: